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ecori sali fragment  (New England Biolabs)


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    New England Biolabs ecori sali fragment
    Ecori Sali Fragment, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 15375 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ecori+sali+fragment/EcoRI/pmc10954446__gkae050_supplemental_file-35-16-72
    Average 99 stars, based on 15375 article reviews
    ecori sali fragment - by Bioz Stars, 2026-10
    99/100 stars

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    Residue:

    Article Title: Molecular Basis for Unidirectional Scaffold Switching of Human Plk4 in Centriole Biogenesis
    Article Snippet: .. To express MBPL (a longer form of MBP with a 30 residue insertion)-fused Cep192-58mer, a EcoRI-SalI fragment containing the MBP C-terminal 30 residue-TEV-Cep192-58mer from pKM4371 was cloned into the pMAL-C2 vector (New England Biolabs) digested by the same enzymes. .. MBPL-fused Cep192-58mer migrates markedly slower than MBP-fused Cep152-60mer, thus allowing us to differentiate these two proteins in SDS-PAGE. pGEX-4T3-Plk4 C (580–970) (pKM3584) was cloned by inserting a SalI-NotI fragment into the pGEX-4T3 vector (Amersham Pharmacia) digested by the same enzymes. pET21b-Cep192 (201–310) (pKM3619) was generated by inserting a NotI-SalI fragment into the pET21b vector (Novagen) digested by the corresponding enzymes.

    Article Title: Molecular Basis for Unidirectional Scaffold Switching of Human Plk4 in Centriole Biogenesis
    Article Snippet: .. To express MBPL (a longer form of MBP with a 30 residue insertion)-fused Cep192-58mer, a EcoRI-SalI fragment containing the MBP C-terminal 30 residue-TEV-Cep192-58mer from pKM4371 was cloned into the pMAL-C2 vector (New England Biolabs) digested by the same enzymes. .. MBPL-fused Cep192-58mer migrates markedly slower than MBP-fused Cep152-60mer, thus allowing us to differentiate these two proteins in SDS-PAGE. pGEX-4T3-Plk4 C (580–970) (pKM3584) was cloned by inserting a SalI-NotI fragment into the pGEX-4T3 vector (Amersham Pharmacia) digested by the same enzymes. pET21b-Cep192 (201–310) (pKM3619) was generated by inserting a NotI-SalI fragment into the pET21b vector (Novagen) digested by the corresponding enzymes.

    Clone Assay:

    Article Title: Molecular Basis for Unidirectional Scaffold Switching of Human Plk4 in Centriole Biogenesis
    Article Snippet: .. To express MBPL (a longer form of MBP with a 30 residue insertion)-fused Cep192-58mer, a EcoRI-SalI fragment containing the MBP C-terminal 30 residue-TEV-Cep192-58mer from pKM4371 was cloned into the pMAL-C2 vector (New England Biolabs) digested by the same enzymes. .. MBPL-fused Cep192-58mer migrates markedly slower than MBP-fused Cep152-60mer, thus allowing us to differentiate these two proteins in SDS-PAGE. pGEX-4T3-Plk4 C (580–970) (pKM3584) was cloned by inserting a SalI-NotI fragment into the pGEX-4T3 vector (Amersham Pharmacia) digested by the same enzymes. pET21b-Cep192 (201–310) (pKM3619) was generated by inserting a NotI-SalI fragment into the pET21b vector (Novagen) digested by the corresponding enzymes.

    Article Title: Two birds with one stone: SGI1 can stabilize itself and expel the IncC helper by hijacking the plasmid parABS system
    Article Snippet: The amplicon was digested with EcoRI-BamHI and cloned into the EcoRI-BamHI site of pJKI88. pGMY65: The EcoRI-SalI fragment of pGMY64 was inserted into the EcoRI-SalI site of pJKI298 (10). pGMY66: The IGR was deleted from pGMY64 by HincII digestion and religation. pGMY67: The HincII fragment (IGR) of pGMY64 was ligated into the EcoRV site of pBR322 (16). pGMY69: The Km R cassette of pUC4K was cloned into the EcoRI site of pBelobac11 (New England Biolabs). pGMY72: The EcoRI-NotI fragment of pGMY65 was inserted into the EcoRI-NotI site of pBelobac11. pGMY74: The DRnNCfor and DRnNCrev primers containing the DR2 sequence (13371-13401 bp of SGI1) joined to an inverted repeat motif in the IGR (15076-15097 bp of SGI1) were annealed and cloned into the EcoRI-PstI site of pEMBL19. pGMY86: The orf 053 was knocked out in pJKI1128 by self-ligation of blunted ends after BglII digestion and filling in the sticky ends using Klenow polymerase. pGMY91: The 14768-15139 bp region of SGI1 was amplified with primers EcoRIDRHincIINCfor and NC6_Pstrev. .. The amplicon was digested with EcoRI-BamHI and cloned into the EcoRI-BamHI site of pJKI88. pGMY65: The EcoRI-SalI fragment of pGMY64 was inserted into the EcoRI-SalI site of pJKI298 (10). pGMY66: The IGR was deleted from pGMY64 by HincII digestion and religation. pGMY67: The HincII fragment (IGR) of pGMY64 was ligated into the EcoRV site of pBR322 (16). pGMY69: The Km R cassette of pUC4K was cloned into the EcoRI site of pBelobac11 (New England Biolabs). pGMY72: The EcoRI-NotI fragment of pGMY65 was inserted into the EcoRI-NotI site of pBelobac11. pGMY74: The DRnNCfor and DRnNCrev primers containing the DR2 sequence (13371-13401 bp of SGI1) joined to an inverted repeat motif in the IGR (15076-15097 bp of SGI1) were annealed and cloned into the EcoRI-PstI site of pEMBL19. pGMY86: The orf 053 was knocked out in pJKI1128 by self-ligation of blunted ends after BglII digestion and filling in the sticky ends using Klenow polymerase. pGMY91: The 14768-15139 bp region of SGI1 was amplified with primers EcoRIDRHincIINCfor and NC6_Pstrev. ..

    Article Title: Molecular Basis for Unidirectional Scaffold Switching of Human Plk4 in Centriole Biogenesis
    Article Snippet: .. To express MBPL (a longer form of MBP with a 30 residue insertion)-fused Cep192-58mer, a EcoRI-SalI fragment containing the MBP C-terminal 30 residue-TEV-Cep192-58mer from pKM4371 was cloned into the pMAL-C2 vector (New England Biolabs) digested by the same enzymes. .. MBPL-fused Cep192-58mer migrates markedly slower than MBP-fused Cep152-60mer, thus allowing us to differentiate these two proteins in SDS-PAGE. pGEX-4T3-Plk4 C (580–970) (pKM3584) was cloned by inserting a SalI-NotI fragment into the pGEX-4T3 vector (Amersham Pharmacia) digested by the same enzymes. pET21b-Cep192 (201–310) (pKM3619) was generated by inserting a NotI-SalI fragment into the pET21b vector (Novagen) digested by the corresponding enzymes.

    Amplification:

    Article Title: Two birds with one stone: SGI1 can stabilize itself and expel the IncC helper by hijacking the plasmid parABS system
    Article Snippet: The amplicon was digested with EcoRI-BamHI and cloned into the EcoRI-BamHI site of pJKI88. pGMY65: The EcoRI-SalI fragment of pGMY64 was inserted into the EcoRI-SalI site of pJKI298 (10). pGMY66: The IGR was deleted from pGMY64 by HincII digestion and religation. pGMY67: The HincII fragment (IGR) of pGMY64 was ligated into the EcoRV site of pBR322 (16). pGMY69: The Km R cassette of pUC4K was cloned into the EcoRI site of pBelobac11 (New England Biolabs). pGMY72: The EcoRI-NotI fragment of pGMY65 was inserted into the EcoRI-NotI site of pBelobac11. pGMY74: The DRnNCfor and DRnNCrev primers containing the DR2 sequence (13371-13401 bp of SGI1) joined to an inverted repeat motif in the IGR (15076-15097 bp of SGI1) were annealed and cloned into the EcoRI-PstI site of pEMBL19. pGMY86: The orf 053 was knocked out in pJKI1128 by self-ligation of blunted ends after BglII digestion and filling in the sticky ends using Klenow polymerase. pGMY91: The 14768-15139 bp region of SGI1 was amplified with primers EcoRIDRHincIINCfor and NC6_Pstrev. .. The amplicon was digested with EcoRI-BamHI and cloned into the EcoRI-BamHI site of pJKI88. pGMY65: The EcoRI-SalI fragment of pGMY64 was inserted into the EcoRI-SalI site of pJKI298 (10). pGMY66: The IGR was deleted from pGMY64 by HincII digestion and religation. pGMY67: The HincII fragment (IGR) of pGMY64 was ligated into the EcoRV site of pBR322 (16). pGMY69: The Km R cassette of pUC4K was cloned into the EcoRI site of pBelobac11 (New England Biolabs). pGMY72: The EcoRI-NotI fragment of pGMY65 was inserted into the EcoRI-NotI site of pBelobac11. pGMY74: The DRnNCfor and DRnNCrev primers containing the DR2 sequence (13371-13401 bp of SGI1) joined to an inverted repeat motif in the IGR (15076-15097 bp of SGI1) were annealed and cloned into the EcoRI-PstI site of pEMBL19. pGMY86: The orf 053 was knocked out in pJKI1128 by self-ligation of blunted ends after BglII digestion and filling in the sticky ends using Klenow polymerase. pGMY91: The 14768-15139 bp region of SGI1 was amplified with primers EcoRIDRHincIINCfor and NC6_Pstrev. ..

    Sequencing:

    Article Title: Two birds with one stone: SGI1 can stabilize itself and expel the IncC helper by hijacking the plasmid parABS system
    Article Snippet: The amplicon was digested with EcoRI-BamHI and cloned into the EcoRI-BamHI site of pJKI88. pGMY65: The EcoRI-SalI fragment of pGMY64 was inserted into the EcoRI-SalI site of pJKI298 (10). pGMY66: The IGR was deleted from pGMY64 by HincII digestion and religation. pGMY67: The HincII fragment (IGR) of pGMY64 was ligated into the EcoRV site of pBR322 (16). pGMY69: The Km R cassette of pUC4K was cloned into the EcoRI site of pBelobac11 (New England Biolabs). pGMY72: The EcoRI-NotI fragment of pGMY65 was inserted into the EcoRI-NotI site of pBelobac11. pGMY74: The DRnNCfor and DRnNCrev primers containing the DR2 sequence (13371-13401 bp of SGI1) joined to an inverted repeat motif in the IGR (15076-15097 bp of SGI1) were annealed and cloned into the EcoRI-PstI site of pEMBL19. pGMY86: The orf 053 was knocked out in pJKI1128 by self-ligation of blunted ends after BglII digestion and filling in the sticky ends using Klenow polymerase. pGMY91: The 14768-15139 bp region of SGI1 was amplified with primers EcoRIDRHincIINCfor and NC6_Pstrev. .. The amplicon was digested with EcoRI-BamHI and cloned into the EcoRI-BamHI site of pJKI88. pGMY65: The EcoRI-SalI fragment of pGMY64 was inserted into the EcoRI-SalI site of pJKI298 (10). pGMY66: The IGR was deleted from pGMY64 by HincII digestion and religation. pGMY67: The HincII fragment (IGR) of pGMY64 was ligated into the EcoRV site of pBR322 (16). pGMY69: The Km R cassette of pUC4K was cloned into the EcoRI site of pBelobac11 (New England Biolabs). pGMY72: The EcoRI-NotI fragment of pGMY65 was inserted into the EcoRI-NotI site of pBelobac11. pGMY74: The DRnNCfor and DRnNCrev primers containing the DR2 sequence (13371-13401 bp of SGI1) joined to an inverted repeat motif in the IGR (15076-15097 bp of SGI1) were annealed and cloned into the EcoRI-PstI site of pEMBL19. pGMY86: The orf 053 was knocked out in pJKI1128 by self-ligation of blunted ends after BglII digestion and filling in the sticky ends using Klenow polymerase. pGMY91: The 14768-15139 bp region of SGI1 was amplified with primers EcoRIDRHincIINCfor and NC6_Pstrev. ..



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